Journal: Stem Cell Reports

Article Title: Ribosome biogenesis controls cranial suture MSC fate via the complement pathway in mouse and human iPSC models

doi: 10.1016/j.stemcr.2023.10.015

Figure Lengend Snippet: Snord118 depletion leads to MSC p53 activation, cell death, proliferation reduction, and loss of mesenchymal stromal cells Gli1-Cre ERT2 ; Ai-14 mice were used to genetically label Gli1 + suture MSCs, followed by confocal imaging of sagittal sutures after IHC staining. Hoechst stains nuclei (blue). (A and B) IHC staining of Gli1-tdTomato suture MSCs with or without co-staining p53. (C and D) Quantification of the percentage of Gli1-tdTomato + cells and p53 + ;Gli1-tdTomato + cells in the suture mesenchyme per section. (E and F) TUNEL and Ki67 IHC staining cells with or without Gli1-tdTomato. (G and H) Quantification of the percentage of TUNEL- or Ki67-positive Gli1-tdTomato + cells per section. (I and J) IHC staining of Pdgfrα, CD31, and TUBB3. (K–M) Quantification of the percentage of Pdgfrα + , CD31 + , and TUBB3 + cells per section. Scale bars, 100 μm. Values represent mean ± SEM with statistical significance assessed by Student’s t tests (n = 3–4 mice). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns represents nonsignificant.

Article Snippet: An EdU assay was performed by using the EdU Cell Proliferation Kit (Click Chemistry Tools); 10 μM EdU was used to treat cells for 2 h before fixing the samples.

Techniques: Activation Assay, Imaging, Immunohistochemistry, Staining, TUNEL Assay

Journal: Cell reports

Article Title: Cyclin E/CDK2 and feedback from soluble histone protein regulate the S phase burst of histone biosynthesis

doi: 10.1016/j.celrep.2023.112768

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Click-iT TM EdU Cell Proliferation Kit for Imaging , Fisher Scientific , C10340.

Techniques: Recombinant, ISH Cell Assay, Imaging, Software, Hybridization